ab 2315387  (Developmental Studies Hybridoma Bank)

Journal: Cell Genomics

Article Title: ABCA7-dependent induction of neuropeptide Y is required for synaptic resilience in Alzheimer’s disease through BDNF/NGFR signaling

doi: 10.1016/j.xgen.2024.100642

Figure Lengend Snippet: abca7 is required for synaptic integrity, microglial prevalence, and astroglial proliferation in zebrafish (A) Schematic view of generating abca7 knockout line. (B) 44.5-kb deletion in abca7 gene. (C) Representative genotyping PCR gel from F4 generation where positive bands indicate the deletion. Every column is one adult fin-clip DNA sample amplified with the forward and reverse primers in (B). (D) Genotyping results with genomic DNA qPCR in F3 adults. Eight animals (four wild-type and four heterozygous deletions) were used. Heterozygous deletions show reduced amplification (lower fluorescence). Results depicted as violin plots and individual values. (E) Immunofluorescence (IF) for SV2 (green) and L-plastin (red) with DAPI counterstain in wild type and abca7 +/− with and without Aβ42. Black and white panels indicate individual fluorescent channels. (F) IF for PCNA (green) and GS (red) with DAPI counterstain in wild type and abca7 +/− with and without Aβ42. Black and white panels indicate individual fluorescent channels. (G) Quantification of SV2-positive synaptic puncta, number of microglial cells, and number of proliferating astroglia. One-way ANOVA with Tukey’s multiple comparison test was used for statistical analyses. n = 4 animals from both sexes with 24 brain sections per group. ∗ p < 0.0332, ∗∗ p < 0.0021, ∗∗∗ p < 0.0002, ∗∗∗∗ p < 0.0001; not significant (ns), p > 0.0332. Scale bars, 100 μm. See also Figures S1 and , , and Table S1 .

Article Snippet: SV2 , Developmental Studies Hybridoma Bank - DSHB , Cat# SV2; RRID: AB_2315387.

Techniques: Knock-Out, Amplification, Fluorescence, Immunofluorescence, Comparison

Journal: Cell Genomics

Article Title: ABCA7-dependent induction of neuropeptide Y is required for synaptic resilience in Alzheimer’s disease through BDNF/NGFR signaling

doi: 10.1016/j.xgen.2024.100642

Figure Lengend Snippet: ABCA7 regulates synaptic integrity and astroglial proliferation through BDNF (A) Experimental setup for investigating the biological relevance of NPY to abca7 function and amyloid-induced alterations in synaptic integrity, microglial activity, and astroglial proliferation. (B) Immunofluorescence for SV2 + L-plastin and GS + PCNA with DAPI counterstains in abca7 +/− + PBS, abca7 +/− + Aβ42, abca7 +/− + NPY, and abca7 +/− + Aβ42 + NPY brains. (C) Quantification for number of SV2-positive synaptic puncta, L-plastin-positive microglia, and GS/PCNA double-positive proliferating astroglia. n = 4 animals from both sexes and ≥20 brain sections per group. One-way ANOVA with Tukey’s multiple comparison test was used for statistical analyses. No comparison bar indicates no significance. (D) BDNF immunoreactivity in wild-type and abca7 knockout zebrafish with and without Aβ42. Aβ42 induced BDNF (red) in wild-type animals but not in abca7 knockout. Injection of NPY with or without Aβ42 restores the induced BDNF expression levels. Non-parametric Kolmogorov-Smirnov test comparing cumulative distributions and one-way ANOVA with Tukey’s multiple comparison test were used for statistical analyses. (E) Immunofluorescence for SV2 and L-plastin with DAPI counterstain in abca7 +/− + Aβ42 + PBS and abca7 +/− + Aβ42 + BDNF zebrafish brains. Quantification for the number of SV2-positive synaptic puncta and the number of L-plastin-positive microglia. Non-parametric Kolmogorov-Smirnov test comparing cumulative distributions was used for statistical analyses. BDNF rescues synaptic integrity changes in abca7 +/− knockout similar to NPY. n = 4 animals from both sexes, with 18 brain sections per group. ∗ p < 0.0332, ∗∗ p < 0.0021, ∗∗∗ p < 0.0002, ∗∗∗∗ p < 0.0001; not significant (ns), p > 0.0332. Scale bars, 50 μm. See also Figures S1 and , and , and Table S1 .

Article Snippet: SV2 , Developmental Studies Hybridoma Bank - DSHB , Cat# SV2; RRID: AB_2315387.

Techniques: Activity Assay, Immunofluorescence, Comparison, Knock-Out, Injection, Expressing

Journal: Cell Genomics

Article Title: ABCA7-dependent induction of neuropeptide Y is required for synaptic resilience in Alzheimer’s disease through BDNF/NGFR signaling

doi: 10.1016/j.xgen.2024.100642

Figure Lengend Snippet: ABCA7-dependent NPY acts through BDNF/NGFR axis (A) Immunofluorescence for SV2/L-plastin and GS/PCNA with DAPI counterstain in abca7 +/− knockout brains injected with vehicle (PBS), BDNF, and BDNF + NGFR inhibitor. (B) Quantification graphs for SV2 + puncta, number of PCNA/GS double-positive astroglia, and number of L-plastin + microglia. Inhibition of NGFR abrogates the rescue effects of BDNF on synaptic integrity and astroglial proliferation. Two-way ANOVA with Tukey’s multiple comparison test was used for statistical analyses. n = 4 animals from both sexes, with 18 brain sections per group. (C) Immunofluorescence in npy −/− knockout animals for SV2 and BDNF with DAPI nuclear counterstain. (D) Quantification of the number of SV2+ synaptic puncta and BDNF intensity. npy −/− knockout independently reduces BDNF expression and the synaptic integrity. n = 3 animals from both sexes and 18 brain sections per group. (E) Activity model for ABCA7: ABCA7-dependent induction of NPY after amyloid toxicity is required for expression of BDNF, which—through NGFR—regulates the synaptic integrity and astroglial proliferation. ABCA7 function is relevant to brain resilience to AD in an NPY-dependent manner. ∗ p < 0.0332, ∗∗ p < 0.0021, ∗∗∗ p < 0.0002, ∗∗∗∗ p < 0.0001; not significant (ns), p > 0.0332. Scale bars, 50 μm. See also Figures S1 and , and , and Table S1 .

Article Snippet: SV2 , Developmental Studies Hybridoma Bank - DSHB , Cat# SV2; RRID: AB_2315387.

Techniques: Immunofluorescence, Knock-Out, Injection, Inhibition, Comparison, Expressing, Activity Assay

Journal: iScience

Article Title: Loss of the polarity protein Par3 promotes dendritic spine neoteny and enhances learning and memory

doi: 10.1016/j.isci.2024.110308

Figure Lengend Snippet:

Article Snippet: Anti-SV2 , DSHB , Cat#: SV2-S; RRID: AB_2315387.

Techniques: Virus, Recombinant, Activation Assay, Transfection, Phospho-proteomics, Mutagenesis, Software, Plasmid Preparation, Protease Inhibitor, Labeling

Journal: Cell reports

Article Title: Human photoreceptors switch from autonomous axon extension to cell-mediated process pulling during synaptic marker redistribution

doi: 10.1016/j.celrep.2022.110827

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse IgG1 anti-SV2A , Developmental Studies Hybridoma Bank , CatSV2-s; RRID:AB_2315387.

Techniques: Recombinant, Generated, Software

Journal: Cell reports

Article Title: Human photoreceptors switch from autonomous axon extension to cell-mediated process pulling during synaptic marker redistribution

doi: 10.1016/j.celrep.2022.110827

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse IgG1 anti-SV2A , Developmental Studies Hybridoma Bank , CatSV2-s; RRID:AB_2315387.

Techniques: Recombinant, Generated, Software